Free cationic liposomes inhibit the inflammatory response to cationic lipid–DNA complex injected intravenously and enhance its transfection efficiency
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چکیده
منابع مشابه
Asialoganglioside enhances the efficiency of gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
We investigated the transfection efficiency mediated by asialoganglioside-containing cationic liposomes. Previously we reported that monosialoganglioside GM(1) (GM(1a)) enhanced transfection efficiency. In this study, we investigated the effects of sialic acid in gangliosides on transfection efficiency. Two mammalian culture cell lines HeLa and HepG2 were transfected with luciferase plasmids (p...
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PURPOSE Cationic liposomes (CLs) are composed of phospholipid bilayers. One of the most important applications of these particles is in drug and gene delivery. However, using CLs to deliver therapeutic nucleic acids and drugs to target organs has some problems, including low transfection efficiency in vivo. The aim of this study was to develop novel CLs containing magnetite to overcome the defi...
متن کاملBiophysical and transfection properties of DNA-DiC14-amidine complexes.
DiC14-amidine (N-t-butyl-N°-tetradecylaminopropionamidine) is a cationic lipid used as transfection agent in vitro [1] and as a vector for in vivo gene therapy applications. DiC14-amidine molecules assemble in a liposomal structure (transition temperature: 23C-critical micelle concentration: 10-7 M). Compared to other cationic lipids, diC14-amidine does not require a helper lipid. This property...
متن کاملCationic Liposomes Modified with Polyallylamine as a Gene Carrier: Preparation, Characterization and Transfection Efficiency Evaluation.
Purpose: Cationic polymers and cationic liposomes have shown to be effective non-viral gene delivery vectors. In this study, we tried to improve the transfection efficiency by employing the advantages of both. Methods: For this purpose, modified polyallylamines (PAAs) were synthesized. These modifications were done through the reaction of PAA (15 KDa) with acrylate and 6-bromoalkanoic acid deri...
متن کاملThe Effect of Linear PEI on Characteristics and Transfection Efficiency of PEI-Based Cationic Nanoliposomes
The development of efficient and safe carrier system to transfer DNA into cells is essential in non-viral gene therapy. The aim of the present study was to evaluate the effect of linear polyetheneimine (lPEI) (2500 Da) on the physicochemical and biological properties of lipopolyplexes constructed from liposomes and lPEI. Materials and Methods Different lipopolymers were synthesized from lPEI ...
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ژورنال
عنوان ژورنال: Molecular Therapy
سال: 2003
ISSN: 1525-0016
DOI: 10.1016/s1525-0016(02)00032-1